Independently of the chosen nucleic acid labeling scheme, we strongly recommend validation experiments as shown in Fig.
Other modified nucleosides are shown in Fig.
A +7 mass increase is observed for cytidine and uridine and a +5 and +4 mass increase for adenosine and guanosine, respectively.
The promising results from the validation experiments allowed the design of pulse chase experiments.
In comparison to the natural abundance of these modified nucleosides ~0.
Other modified nucleosides such as Cm, Gm, and the base-methylated G derivatives m 1G, m 2G, and m 22G are incorporated more slowly and the final modification density is not reached within 48 h.